Sexuality researchers from a range of disciplines have called for more global inclusiveness in sexualities research, particularly in the Global South (GS). We investigated the degree to which sexuality researchers have published work focused on the GS by conducting a content analysis of 50years of research published in the Journal of Sex Research (JSR). We examined all research articles, brief research reports, and clinical notes published in JSR from 1965 to 2014 (N=1,626). Overall, a small percentage of articles focused on the GS with no increase over time (4.8%; N=78). Articles in the GS focused on Asia (37.2%), Latin America (28.2%), Sub-Saharan Africa (23.1%), the Middle East (6.4%), and a mix of GS regions (5.1%). Topics related to sexual and reproductive health were most prevalent (19.2%), followed by articles on sexual risk, sexually transmitted infections (STIs), and human immunodeficiency virus (HIV) (17.9%), sexual norms, attitudes, and beliefs (16.7%), sex work (11.5%), cultural practices (10.3%), gay, lesbian, and bisexual issues (9.0%), intimate partner relationships (3.8%), and sexual violence (3.8%); the remaining categories (transgender and transsexual populations, sex research methods, sex testing, and women's sexuality) were negligible. We conclude with recommendations for improving the quantity, quality, and scope of global sexuality research in JSR.