Rust disease is an important disease on soybean. It caused yield losses, up to 85%. The best control measured was planting the resistant varieties. The resistant gens can be obtain from the germplasm collection. Evaluation of the resistant soybean germplasm collection infected rust disease was done at Kendalpayak Experimental Station, Indonesian Legumes and Tuber Crops Research Institute (ILETRI), from June to September 2013, with 225 genotypes of soybean germplasm collection. Besides, at green house of Plant Protection Unit in ILETRI, from March to May 2014, with 50 genotypes that have already screened in the field. Observation of rust disease resistance based on the International Working Group on Soybean Rust system. Out of 225 soybean genotypes tested there was not any resistant one, 218 genotypes were moderate resistant, and 7 genotypes were moderate susceptible to rust disease in the field. There was not any resistant one, 8 genotypes were moderate resistant, 33 genotypes were moderate susceptible, and 9 genotypes were susceptible to rust disease in the green house. Eight soybeans accession had consistent result in the green house and the field, there were MLG 0852, MLG 0887, MLG 937, MLG 1024, MLG 1034, MLG 1035, MLG 1065, MLG 1066.