Electrocatalysis of β-nicotinamide adenine dinucleotide oxidation with electropolymerized films of toluidine blue

A stable electropolymerized toduiline blue films are deposited on the glassy carbon by the method of cyclic voltammetry, their electrochemical quality is studied extensively in 0.2 mol/L phosphate buffer (pH 6. 86) and their electrochemical behavior is discussed clearly in the presence of various pH values. In this paper, electrocatalysis of beta-nicotinamide adenine dinucleotide (NADH) oxidation with the poly-toduiline blue films is checked by the method of cyclic voltammetry and the catalytic reaction constant between NADH and poly-toduiline blue is determined by rotation disc electrode measurement. The experiments proved that the poly-toduiline blue is an excellent catalyst for NADH oxidation, which peak potential shifit negatively to 450 mV on glassy carbon electrode modified with the poly-toduiline blue films compared with unmodified glassy carbon electrode, and the reaction constant of NADH oxidation on the glassy carbon electrode modified with the polytoduiline blue films is exponentially greater that on modified glassy carbon electrode.