Application of GC and GC-MS for the identification of fatty acids in Absidia coerulea

The paper presents the study on the content and composition of lipids in the saprophytic fungus of forest soil - Absidia cocrulea 93. Both free fatty acids (FFAs) and fatty acids being a part of triacylglycerols have been characterized. High Performance Liquid Chromatography (HPLC) was employed to separate different classes of compounds from the fungus extract, whereas Gas Chromatography (GC) and Mass Spectrometry combined with Gas Chromatography (GC-MS) were used for separation and identification of the individual compounds. Length of chains of the fatty acids, both FFAs and in triacylglycerols, ranged from C-14 to C-24. In both cases two C-18 acids were dominant: oleic and palmitic.