An alkaline protease inhibitor from Aspergillus oryzae W-1

被引：0

作者：

Ishihara, M ^{[1
]}

Katayama, H ^{[1
]}

Taira, T ^{[1
]}

Tawata, S ^{[1
]}

Kobamoto, N ^{[1
]}

机构：

[1] Univ Ryukyus, Fac Agr, Dept Biosci & Biotechnol, Nishihara, Okinawa 9030213, Japan

来源：

JOURNAL OF THE JAPANESE SOCIETY FOR FOOD SCIENCE AND TECHNOLOGY-NIPPON SHOKUHIN KAGAKU KOGAKU KAISHI | 2003年 / 50卷 / 07期

关键词：

D O I：

暂无

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

An alkaline protease inhibitor (API) from Aspergillus oryzae W-1 was purified to homogeneity by DEAE-Cellulose and Hvdroxyl apatite column chromategraphies. The molecular mass of the inhibitor was estimated to be 14 KDa by SDS-PAGE and 12.5KDa by gel filtration on Sephadex G-50 column chromatography; the isoelectric point was 4.6. API was extremely heat stable and retained 100% of its original activity even after heating in a boiling water bath for 5 min in the pH range of 4-6. API exhibited an inhibitory activity against the proteolytic activity of alkaline protease from A. oryzae or A. oryzae W-1 but not for subtilisin, subtilisin BPN', papain. ficin. bromelain, trypsin and a-chymotrypsin. It was found that the inhibitor was inactivated by the action of the latter seven enzymes.

引用

页码：327 / 330

页数：4

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