INFLUENCE OF CULTURE CONDITIONS ON LIGNINOLYTIC ENZYMES PRODUCTION FROM Ganoderma lucidum USING AGRO-INDUSTRIAL RESIDUES

The present study was carried out to investigate the ligninolytic enzymes production potential of G. lucidum using different agro-industrial residues and their by-products. The enzyme profile of G. lucidum showed highest activities of manganese peroxidase (MnP) after 4th day of incubation on peanut shells followed by lignin peroxidase (LiP) and laccase after 6th and 7th day of incubation on orange peel and wheat straw, respectively. The enzyme with maximum activity (MnP) was optimized, therefore, through process optimization the production of MnP was substantially enhanced up to 2160 +/- 212 U/mL, when fermentation medium of peanut shells inoculated with 5 mL spore suspension of G. lucidum. Other optimum conditions were: pH, 5; moisture level, 70%; temperature, 40 degrees C; carbon source, glucose (1%); nitrogen supplement, yeast extract (0.2%), and modulator, MnSO4.