Paclitaxel and baccatin III production induced by methyl jasmonate in free and immobilized cells of Taxus baccata

被引：24
|
作者：
Bonfill, M.
Bentebibel, S.
Moyano, E.
Palazon, J.
Cusido, R. M.
Eibl, R.
Pinol, M. T.
机构：
[1] Univ Barcelona, Fac Farm, Lab Fisiol Vegetal, E-08028 Barcelona, Spain
[2] Hsch Wadenswill, CH-8820 Wadenswil, Switzerland
[3] Univ Pompeu Fabra, Dept Ciencies Expt & Salut, E-08003 Barcelona, Spain
来源：
BIOLOGIA PLANTARUM | 2007年 / 51卷 / 04期
关键词：
alginate entrapped cells; cell cultures; elicitation; taxanes; yew;
D O I：
10.1007/s10535-007-0137-2
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
The effects of 100 and 200 mu M methyl jasmonate (MJA) on cell proliferation and paclitaxel and baccatin III production were investigated in free and alginate immobilized cells of Taxus baccata growing in a selected product formation culture medium. The greatest accumulation of paclitaxel (13.20 mg dm(-3)) and baccatin III (4.62 mg dm(-3)) occurred when 100 mu M MJA was added to the culture medium of cells entrapped using a 1.5 and 2.5 % alginate solution. The effects of different treatments on the viability of cultured cells and their capacity to excrete both taxanes into the surrounding medium were considered.
引用
收藏
页码：647 / 652
页数：6
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