Oxidative Stress Responses in Gills of Tilapia (Oreochromis niloticus) at Different Salinities

The objective of present study is to evaluate the impact of different salinities on the levels of CAT, GSH and MDA of the gills of Nile tilapia (Oreochromis niloticus). Nile tilapia was treated by exposure to salinities concentration 0 parts per thousand, 5 parts per thousand and 10 parts per thousand. Research models were weakened and sacrificed, then took the left and right sides of the gills. The result of gills homogenity was centrifuged for supernatan, then supernatan was proceed with testing levels of CAT, GSH and MDA by ELISA assay methods. The levels of CAT in gills were significantly higher at 10 parts per thousand than at 5 parts per thousand and 0 parts per thousand. The levels of GSH in gills were significantly higher at 0 parts per thousand than 5 parts per thousand. The levels of GSH in gills at 5 parts per thousand and 10 parts per thousand salinities were not significantly different. The levels of MDA in gills at salinity 10 parts per thousand and 5 parts per thousand were higher than in control gills at 0 parts per thousand salinities. This occurs because the salinity of 10 parts per thousand salinity was optimal for live of fish tilapia. In conclusion, salinity impact the increasing of CAT, GSH, and MDA levels in gills of Nile tilapia.