Impact of maternal melatonin suppression on amount and functionality of brown adipose tissue (BAT) in the newborn sheep

被引:38
|
作者
Seron-Ferre, Maria [1 ,2 ]
Reynolds, Henry [1 ,2 ]
Andrea Mendez, Natalia [3 ]
Mondaca, Mauricio [1 ,2 ]
Valenzuela, Francisco [1 ,2 ]
Ebensperger, Renato [1 ,2 ]
Valenzuela, Guillermo J. [4 ]
Herrera, Emilio A. [2 ]
Llanos, Anibal J. [2 ]
Torres-Farfan, Claudia [3 ]
机构
[1] Univ Chile, Inst Ciencias Biomed ICBM, Fac Med, Lab Cronobiol, Santiago, Chile
[2] Univ Chile, Inst Ciencias Biomed ICBM, Fac Med, Programa Fisiopatol, Santiago, Chile
[3] Univ Austral Chile, Fac Med, Lab Cronobiol Desarrollo, Valdivia, Chile
[4] Arrowhead Reg Med Ctr, Dept Womens Hlth, San Bernardino, CA USA
来源
FRONTIERS IN ENDOCRINOLOGY | 2015年 / 5卷
关键词
development; constant light; circadian; SCN; thermoregulation; newborn; CLOCK GENE-EXPRESSION; OVINE FETUS; SUMMIT METABOLISM; PLASMA-GLUCOSE; BINDING-SITES; ADRENAL-GLAND; FETAL SHEEP; PPAR-GAMMA; RECEPTORS; CATECHOLAMINES;
D O I
10.3389/fendo.2016.00232
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
In human and sheep newborns, brown adipose tissue (BAT) accrued during fetal development is used for newborn thermogenesis. Here, we explored the role of maternal melatonin during gestation on the amount and functionality of BAT in the neonate. We studied BAT from six lambs gestated by ewes exposed to constant light from 63% gestation until delivery to suppress melatonin (LL), six lambs gestated by ewes exposed to LL but receiving daily oral melatonin (12 mg at 1700 h, LL + Mel) and another six control lambs gestated by ewes maintained in 12 h light:12 h dark (LD). Lambs were instrumented at 2 days of age. At 4-6 days of age, they were exposed to 24 degrees C (thermal neutrality conditions) for 1 h, 4 degrees C for 1 h, and 24 degrees C for 1 h. Afterward, lambs were euthanized and BAT was dissected for mRNA measurement, histology, and ex vivo experiments. LL newborns had lower central BAT and skin temperature under thermal neutrality and at 4 degrees C, and higher plasma norepinephrine concentration than LD newborns. In response to 4 degrees C, they had a pronounced decrease in skin temperature and did not increase plasma glycerol. BAT weight in LL newborns was about half of that of LD newborns. Ex vivo, BAT from LL newborns showed increased basal lipolysis and did not respond to NE. In addition, expression of adipogenic/thermogenic genes (UCP1, ADBR3, PPARy, PPARa, PGC1a, C/EBP1, and perilipin) and of the clock genes Bma11, Clock, and Per2 was increased. Remarkably, the effects observed in LL newborns were absent in LL + Mel newborns. Thus, our results support that maternal melatonin during gestation is important in determining amount and normal functionality of BAT in the neonate.
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页数:11
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