Microchip Electrophoresis Using Linear-Imaging UV Detector

被引：0
|
作者：
Kitagawa, Fumihiko [1 ]
Aizawa, Shiori [1 ]
Otsuka, Koji [1 ]
机构：
[1] Kyoto Univ, Grad Sch Engn, Dept Chem Mat, Nishikyo Ku, Kyoto 6158510, Japan
来源：
BUNSEKI KAGAKU | 2011年 / 60卷 / 09期
关键词：
microchip electrophoresis; cyclodextrin electrokinetic chromatography; isoelectric focusing; linear-imaging UV detector; CAPILLARY-ELECTROPHORESIS; SYSTEMS;
D O I：
10.2116/bunsekikagaku.60.725
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
We have studied on microchip-cyclodextrin electrokinetic chromatography (MC-CDEKC) analysis of enantiomers and microchip isoelectric focusing (MC-IEF) of proteins by using a commercially available microchip electrophoresis (MCE) instrument equipped with a linear-imaging UV detector, which allows real-time measurements of concentration profiles in a microchannel. In the MC-CDEKC analysis of racemic 1-aminoindane, sulfated-beta-cyclodextrin (S-beta-CD) was used as a pseudo-stationary phase. Effect of the concentration of S-beta-CD on the separation process was investigated. In the MC-IEF analysis of proteins, on the other hand, we studied the processes of a pH gradient formation and protein focusing. Furthermore, effect of the channel length and applied electric field strength on the separation performance of proteins in MC-IEF was studied.
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收藏
页码：725 / 734
页数：10
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