Determination of Heavy Metal in Anchovy (Engraulis encrasicolus L 1758) and Whiting (Merlangius merlangus euxinus Nordman, 1840) Fish in The Middle Black Sea

In this research, the determination of heavy metals in fishes (anchovy and whiting) caught in Samsun which is the province of Middle Black Sea was aimed. Having been washed, dried and cut in to small pieces the fishes HNO3 and HClO4 were added and using wet ash method, heavy metal concentrations were determined. By taking three samples from each fish the concentrations of Fe, Mn, Zn, Cu, Pb, Cd were determined with Flame AAS to be the result of analysis in fishes. In the resulting analysis, in 2009, metal concentration of anchovy have been found 34.0 +/- 2.5 mu g/g for Fe; 2.0 +/- 0.0 mu g/g for Mn; 129.3 +/- 15.0 mu g/g for Zn; 3.7 +/- 1.6 mu g/g for Cu; 0.4 +/- 0.2 mu g/g for Pb; 0.2 +/- 0.05 mu g/g for Cd in 2009, metal concentration of whiting have been found 9.9 +/- 2.1 mu g/g for Fe; 4.3 +/- 0.7 mu g/g for Mn; 58.0 +/- 3.5 mu g/g for Zn; 2.3 +/- 0.7 mu g/g for Cu; 0.9 +/- 0.2 mu g/g for Pb; 0.2 +/- 0.03 mu g/g for Cd in 2010, metal concentration of anchovy have been found 51.5 +/- 5.3 mu g/g for Fe; 4.2 +/- 0.9 mu g/g for Mn; 221.0 +/- 10.5 mu g/g for Zn; 3.8 +/- 1.9 mu g/g for Cu in 2010, metal concentration of whiting have been found 7.0 +/- 4.6 mu g/g for Fe; 3.0 +/- 0.0 mu g/g for Mn; 28.3 +/- 1.0 mu g/g for Zn; 2.7 +/- 0.7 mu g/g for Cu. Anchovies and whiting in the study as a result of heavy metal concentration were high in general. In order to determine the accuracy of the method used in the wet ash method to the reference fish protein samples (DORM-3) was applied.