Protocol for high-throughput cloning, expression , purification, and evaluation of bispecific antibodies

被引：1

作者：

Li, Danqing ^{[1
]}

Partin, Alexander C. ^{[1
]}

Zhao, Liangjun ^{[1
]}

Chen, Irwin ^{[2
]}

Michaels, Mark L. ^{[1
]}

Wang, Zhulun ^{[1
]}

Garces, Fernando ^{[1
]}

Gong, Danyang ^{[1
]}

Riley, Timothy P. ^{[1
]}

机构：

[1] Amgen Inc, Dept Therapeut Discovery, Amgen Res, Thousand Oaks, CA 91320 USA

[2] Amgen Inc, Dept Therapeut Discovery, Amgen Res, San Francisco, CA 94080 USA

来源：

STAR PROTOCOLS | 2022年 / 3卷 / 02期

关键词：

Antibody; Biotechnology and bioengineering; High Throughput Screening; Protein Biochemistry; Protein expression and purification;

D O I：

10.1016/j.xpro.2022.101428

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Bispecific antibodies are a powerful new class of therapeutics, but their develop-ment often requires enormous amounts of time and resources. Here, we describe a high-throughput protocol for cloning, expressing, purifying, and evaluating bis-pecific antibodies. This protocol enables the rapid screening of large panels of bispecific molecules to identify top candidates for further development.For complete details on the use and execution of this protocol, please refer to Estes et al. (2021).

引用

页数：21

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