Fasciola hepatica:: Surface tegumental responses to in vitro and in vivo treatment with the experimental fasciolicide OZ78

The tegumental alterations in adult Fasciola hepatica induced by the experimental fasciolide OZ78 were investigated utilizing scanning electron microscopy (SEM). Twelve weeks post-infection with F hepatica, rats were treated with a single 100 mg/kg oral dose of OZ78 and flukes were recovered from the bile ducts after 24-72 h. In vitro F hepatica were incubated with OZ78 for 48 h at a concentration of 10 mu g/ml in the absence or presence of haemin. Twenty-four and 48 h post-treatment of rats disruption of the tegument of F hepatica as blebbing, swelling and furrowing was evident. The recovery of flukes 72 h post-treatment was low. Flukes examined at this time point showed an increasing severity of tegumental damage as sloughing and absence of spines, in particular in the tail region. SEM analysis of F. hepatica incubated in the presence of OZ78 without haemin showed only minor and localized damage of the tegument. In the presence of haemin extensive tegumental damage, including sloughing or blebbing, in particular in the anterior part, was observed. In conclusion, our experiments confirm the interesting fasciocidal properties of OZ78. The tegument of adult F. hepatica might play a role in the action of this drug. (C) 2008 Elsevier Inc. All rights reserved.