Supramolecular complex formation by β-cyclodextrin and ferrocenylnaphthalene diimide-intercalated double stranded DNA and improved electrochemical gene detection

被引：36

作者：

Sato, S ^{[1
]}

Nojima, T ^{[1
]}

Waki, M ^{[1
]}

Takenaka, S ^{[1
]}

机构：

[1] Kyushu Inst Technol, Dept Mat Sci, Tobata Ku, Kitakyushu, Fukuoka 8048550, Japan

来源：

MOLECULES | 2005年 / 10卷 / 06期

关键词：

ferrocenylnaphthalene diimide; beta-cyclodextrin (CD); DNA; electrochemical detection; supramolecular complex;

D O I：

10.3390/10060693

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ferrocenylnaphthalene diimide 1 can bind to double stranded DNA (dsDNA) by the threading intercalation mode and the resulting complex was stabilized further by beta-cyclodextrin (CD) by forming a supramolecular complex. These complex formation processes were studied by spectroscopic, viscometric, and electrochemical means in the absence or presence of beta-CD. Quantitative analysis by quartz crystal microbalance (QCM) and electrochemical experiments strongly suggested a 2:1 binding stoichiometry for beta-CD to 1 threading-intercalated to the dsDNA-immobilized electrode. Owing to this supramolecular complex formation, electrochemical DNA detection based on 1 was improved considerably.

引用

页码：693 / 707

页数：15

共 7 条

[1] Electrochemical gene detection based on supramolecular complex formation by ferrocenyl-β-cyclodextrin and adamantylnaphthalene diimide bound to double stranded DNA
Sato, S
Nojima, T
Takenaka, S
JOURNAL OF ORGANOMETALLIC CHEMISTRY, 2004, 689 (25) : 4722 - 4728
[2] Pseudo-polyferrocene coating of double stranded DNA with ferrocenylnaphthalene diimide and its application for electrochemical gene detection
Takenaka, S
POLYMER JOURNAL, 2004, 36 (07) : 503 - 512
[3] Pseudo-polyferrocene Coating of Double Stranded DNA with Ferrocenylnaphthalene Diimide and Its Application for Electrochemical Gene Detection
Shigeori Takenaka
Polymer Journal, 2004, 36 : 503 - 512
[4] Analysis of electrochemical reaction of ferrocenylnaphthalene diimide captured by double-stranded DNA during the electrochemical detection of DNA hybridization
Komatsu, M
Nojima, T
Takenaka, S
ELECTROCHEMISTRY, 2006, 74 (01) : 65 - 67
[5] Cooperative Binding of Ferrocenylnaphthalene Diimide Carrying β-Cyclodextrin Converts Double-Stranded DNA to a Rod-Like Structure
Sato, Shinobu
Umeda, Yuta
Fujii, Satoshi
Takenaka, Shigeori
BIOCONJUGATE CHEMISTRY, 2015, 26 (03) : 379 - 382
[6] Cooperative Binding of Ferrocenylnaphthalene Diimide Carrying β-Cyclodextrin Converts Double-Stranded DNA to a Rod-Like Structure (vol 26, pg 379, 2015)
Sato, Shinobu
Umeda, Yuta
Fujii, Satoshi
Takenaka, Shigeori
BIOCONJUGATE CHEMISTRY, 2015, 26 (04) : 790 - 790
[7] Manganese porphyrin-double stranded DNA complex guided in situ deposition of polyaniline for electrochemical thrombin detection
Xie, Shunbi
Chai, Yaqin
Yuan, Yali
Yuan, Ruo
CHEMICAL COMMUNICATIONS, 2014, 50 (54) : 7169 - 7172

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