Purification of PASII/PMP22 - an extremely hydrophobic glycoprotein of PNS myelin membrane

被引：10
|
作者：
Sedzik, J
Kotake, Y
Uyemura, K
机构：
[1] Keio Univ, Sch Med, Dept Physiol, Tokyo 160, Japan
[2] Univ Uppsala, Dept Biochem, S-75123 Uppsala, Sweden
来源：
NEUROREPORT | 1998年 / 9卷 / 07期
关键词：
hydrophobicity; IMAC; membrane proteins; PASII/PMP22; purification;
D O I：
10.1097/00001756-199805110-00062
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
PASII/PMP22 protein (mol. wt 22 kDa, pI 8.8) is an abundant and extremely hydrophobic glycoprotein of PNS myelin which is solubilized effectively by SDS. In humans, this protein is involved in hereditary neuropathies. Here we describe a simple method for purification of PASII/PMP22, suitable for crystallization trials. We usually obtained 10-20 mg PASII/PMP22 from 10 g bovine spinal roots. It is notable, that the original protocol was designated for purification of PO myelin glycoprotein, and purification of PASII/PMP22 is a bonus. Extensive crystallization trials are in progress. (C) 1998 Rapid Science Ltd.
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收藏
页码：1595 / 1600
页数：6
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