Involvement of the N-Terminal Region and Its Characteristic Coiled-Coil Fragment in the Function and Structure Maintenance of E-coli LonA Protease

被引：4
|
作者：
Andrianova, A. G. [1 ]
Kudzhaev, A. M. [1 ]
Dubovtseva, E. S. [1 ]
Rotanova, T. V. [1 ]
机构：
[1] Russian Acad Sci, Shemyakin Ovchinnikov Inst Bioorgan Chem, Ul Miklukho Maklaya 16-10, Moscow 117997, Russia
来源：
RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY | 2017年 / 43卷 / 04期
基金：
俄罗斯科学基金会;
关键词：
AAA(+) proteins; LonA proteases; ATP-dependent proteolysis; role of N-terminal region; ATP-DEPENDENT PROTEASES; QUALITY CONTROL; LIMITED PROTEOLYSIS; DOMAIN; CLASSIFICATION; CHAPERONES; ATPASES; BINDING;
D O I：
10.1134/S1068162017040021
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The truncated form of E. coli LonA protease (EcLon) lacking the N-terminal fragment 1-172 (Lon173) and the variant with deleted coiled-coil (CC) fragment 173-283 (dCC-Lon, a deletion form) are produced and characterized to study the role of the N-terminal region in the functioning of this protease. A comparative analysis of the properties of full-length EcLon protease, dCC-Lon, and Lon173 as well as an earlier produced form with retained C-terminal region (235-280) of CC fragment, Lon235, is performed. As is shown, fragment 1-280 plays an important role in both formation of the ATPase site and maintenance of a stable EcLon protease conformation. Fragment 107-172 is of a paramount importance for implementation of the processive mechanism of ATP-dependent proteolysis.
引用
收藏
页码：368 / 376
页数：9
相关论文
共 28 条
  • [1] Involvement of the N-terminal region and its characteristic coiled-coil fragment in the function and structure maintenance of E. coli LonA protease
    A. G. Andrianova
    A. M. Kudzhaev
    E. S. Dubovtseva
    T. V. Rotanova
    Russian Journal of Bioorganic Chemistry, 2017, 43 : 368 - 376
  • [2] Crystal structure of the N-terminal domain of E-coli Lon protease
    Li, M
    Rasulova, F
    Melnikov, EE
    Rotanova, TV
    Gustchina, A
    Maurizi, MR
    Wlodawer, A
    PROTEIN SCIENCE, 2005, 14 (11) : 2895 - 2900
  • [3] Homotetrameric structure of the SNAP-23 N-terminal coiled-coil domain
    Freedman, SJ
    Song, HK
    Xu, YW
    Sun, ZYJ
    Eck, MJ
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (15) : 13462 - 13467
  • [4] Structure of the N-terminal fragment of Escherichia coli Lon protease
    Li, Mi
    Gustchina, Alla
    Rasulova, Fatima S.
    Melnikov, Edward E.
    Maurizi, Michael R.
    Rotanova, Tatyana V.
    Dauter, Zbigniew
    Wlodawer, Alexander
    ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2010, 66 : 865 - 873
  • [5] Oligomerization of the Fes tyrosine kinase - Evidence for a coiled-coil domain in the unique N-terminal region
    Read, RD
    Lionberger, JM
    Smithgall, TE
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (29) : 18498 - 18503
  • [6] N-Terminal Coiled-Coil Structure of ATPase Subunits of 26S Proteasome Is Crucial for Proteasome Function
    Inobe, Tomonao
    Genmei, Reiko
    PLOS ONE, 2015, 10 (07):
  • [7] The N-terminal coiled-coil domain of Arabidopsis CROWDED NUCLEI 1 is required for nuclear morphology maintenance
    Yin, Chunmei
    Wang, Yuanda
    Wang, Pan
    Chen, Guangxin
    Sun, Aiqing
    Fang, Yuda
    PLANTA, 2024, 260 (03)
  • [8] Structure of the N-terminal coiled-coil domains of the ciliary protein Rpgrip1l
    He, Ran
    Chen, Guanhao
    Li, Zhiwei
    Li, Jianchao
    ISCIENCE, 2023, 26 (03)
  • [9] Solution structure of the N-terminal domain of the delta subunit of the E-coli ATPsynthase
    Wilkens, S
    Dunn, SD
    Chandler, J
    Dahlquist, FW
    Capaldi, RA
    NATURE STRUCTURAL BIOLOGY, 1997, 4 (03) : 198 - 201
  • [10] Comparative study of the N-terminal hydrophilic region in Streptomyces lividans and E-coli FtsY
    Maeda, I
    Hirose, N
    Yamashiro, H
    Ichibori, A
    Ohshima, S
    Fujimoto, T
    Kawase, M
    Yagi, K
    CURRENT MICROBIOLOGY, 2003, 47 (01) : 22 - 25
123