Adhesion strengths between cancer cells with different malignancies and endothelial cells measured using atomic force microscopy

被引：3
|
作者：
Kim, Hyonchol [1 ,2 ]
Ishibashi, Kenta [1 ,2 ]
Okada, Tomoko [1 ]
Nakamura, Chikashi [1 ,2 ]
机构：
[1] Natl Inst Adv Ind Sci & Technol, Biomed Res Inst, 1-1-1 Higashi, Tsukuba, Ibaraki 3058565, Japan
[2] Tokyo Univ Agr & Technol, Dept Biotechnol & Life Sci, 2-24-16 Nakamachi, Koganei, Tokyo 1848588, Japan
来源：
JAPANESE JOURNAL OF APPLIED PHYSICS | 2020年 / 59卷 / SD期
关键词：
QUANTIFICATION; RECEPTORS; SURFACE; SPECTROSCOPY; INVASION; GROWTH; EGFR; AFM;
D O I：
10.7567/1347-4065/ab51ad
中图分类号：
O59 [应用物理学];
学科分类号：
摘要：
Intercellular adhesion strengths of murine breast cancer cells with different malignancies (FP10SC2, highly metastatic and 4T1-LM weakly metastatic) with murine endothelial cells (BMEC) were measured using a cup-attached chip by atomic force microscopy with contact time of the cells between 0 and 60 s. The work required to separate FP10SC2 from a BMEC was larger than that to separate 4T1-LM after short contact times. In addition, cells were greatly elongated at the separation between FP10SC2 and BMEC. From these results, we suggested a model that the adhesion properties of cancer cells measured in this study were representations of detached cancer cells as circulating tumor cells, and highly metastatic cancer cells strongly adhered to endothelial cells with large deformation in floating conditions. These results indicated that our method is useful to study the processes of cancer cell metastasis with respect to intercellular adhesion. (C) 2019 The Japan Society of Applied Physics
引用
收藏
页数：6
相关论文
共 50 条
  • [1] Quantitative Measurements of Intercellular Adhesion Strengths between Cancer Cells with Different Malignancies Using Atomic Force Microscopy
    Kim, Hyonchol
    Ishibashi, Kenta
    Matsuo, Kosuke
    Kira, Atsushi
    Okada, Tomoko
    Watanabe, Kenta
    Inada, Masaki
    Nakamura, Chikashi
    ANALYTICAL CHEMISTRY, 2019, 91 (16) : 10557 - 10563
  • [2] Selective measurement and manipulation of adhesion forces between cancer cells and bone marrow endothelial cells using atomic force microscopy
    Reeves, Kimberley J.
    Hou, Jia
    Higham, Susan E.
    Sun, Zhe
    Trzeciakowski, Jerome P.
    Meininger, Gerald A.
    Brown, Nicola J.
    NANOMEDICINE, 2013, 8 (06) : 921 - 934
  • [3] Docking and fusion sites in human endothelial cells imaged and measured by using atomic force microscopy
    Schneider, SW
    Ossig, R
    Görge, T
    Matzke, R
    Rogge, P
    Niemeyer, A
    Oberleithner, H
    EXPERIMENTAL DERMATOLOGY, 2004, 13 (09) : 580 - 580
  • [4] Dynamic adhesion of T lymphocytes to endothelial cells revealed by atomic force microscopy
    Zhang, Xiaohui
    Wojcikiewicz, Ewa P.
    Moy, Vincent T.
    EXPERIMENTAL BIOLOGY AND MEDICINE, 2006, 231 (08) : 1306 - 1312
  • [5] Computational analysis of adhesion force in the indentation of cells using atomic force microscopy
    Zhang, C. Y.
    Zhang, Y. W.
    PHYSICAL REVIEW E, 2008, 77 (02):
  • [6] Single integrin molecule adhesion forces in intact cells measured by atomic force microscopy
    Lehenkari, PP
    Horton, MA
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1999, 259 (03) : 645 - 650
  • [7] ADHESION DYNAMICS OF BREAST CARCINOMA CELLS TO BONE MARROW ENDOTHELIAL CELLS ASSESSED BY ATOMIC FORCE MICROSCOPY
    Xie, L.
    Sun, Z.
    Hong, Z.
    Brown, N.
    Glinski, O.
    Meininger, G.
    Glinsky, V.
    JOURNAL OF VASCULAR RESEARCH, 2015, 52 : 43 - 43
  • [8] Study on Adhesion Force of Endothelial Progenitor Cells and Endothelial Cells on Different Adhesion Substrates
    Wang, G. X.
    Tang, C. J.
    Xiao, L.
    Wu, X.
    Xie, X.
    Yang, L.
    WORLD CONGRESS ON MEDICAL PHYSICS AND BIOMEDICAL ENGINEERING, VOL 25, PT 10: BIOMATERIALS, CELLULAR AND TISSUE ENGINEERING, ARTIFICIAL ORGANS, 2009, 25 (10): : 143 - 145
  • [9] Comparison of the viscoelastic properties of cells from different kidney cancer phenotypes measured with atomic force microscopy
    Rebelo, L. M.
    de Sousa, J. S.
    Mendes Filho, J.
    Radmacher, M.
    NANOTECHNOLOGY, 2013, 24 (05)
  • [10] Bacterial adhesion measured by atomic force microscopy
    Walker, Asriel
    Peraza, Cindy
    Volle, Catherine
    Ferguson, Megan
    Spain, Eileen
    Nunez, Megan
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2018, 256
12345