We studied microhabitat use and selection by Northern Cricket Frogs (Acris crepitans) along a stream in west-central Missouri. Field observations of microhabitat use indicate that cricket frogs preferentially use moist (muddy) substrates with some proximity to shelter items (e.g, rocks) and the water. Laboratory choice experiments support the importance of substrate moisture and shelter presence. Temperature may also play a role in site selection independent of substrate selection.