Effect of Oxygen Supply on Surfactin Production and Sporulation in Submerged Culture of Bacillus subtilis Y9

Fermentation parameters for surfactin production and sporulation in a submerged culture of Bacillus subtilis Y9 with various oxygen transfer rates in 5 L jar fermenters were investigated. The oxygen-uptake rate (OUR) was positively correlated with volumetric surfactin productivity. When OUR value increased from 0 to 250 s(-1), productivity increased up to 45 mg/L.h; however, no further increase was observed at OUR values above 255 s(-1). The volumetric mass transfer coefficient K(L)a increased with increasing agitation speed. However, a reduction in surfactin production was observed at the highest agitation speed of 500 rpm. Productivity sharply decreased after spore appearance, and remained low until the end of the culture. A mesh-type sparger was installed to generate microsized air bubbles. When the system was operated at 400 rpm with the mesh-type sparger, K(L)a was higher than that at 500 rpm with an original sparger. Under agitation at 400 rpm with the mesh-type sparger, productivity was maintained above 42.3 mg/L.h until 24 h, resulting in the highest surfactin concentration of 875 mg/L. Thus, a mesh-type sparger promotes K(L)a, leading to an increase in productivity.