Development of tissue scaffolds with nanoparticles for bone tissue engineering applications

In this study, a series of gelatin-methacrylate (GelMA)/ Silk fibroin tissue scaffolds with different contents of layered double hydroxides (LDHs) were prepared (J/IF LDH), the effects of Mg/Al LDH nanoparticles on physical, chemical, mechanical and biological properties of scaffolds and their use as bone tissue were investigated. Mg/Al LDH nanoparticles were synthesized by hydrothermal method and characterized by FTIR, XRD, ICP-MS, TEM, particle size and zeta potential analysis. The cytotoxicity of Mg/Al LDH nanoparticles was assesed by using 3,(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay with osteoblast cell line. Mg/Al LDH nanoparticles were found to be non-toxic. J/ IF LDH tissue scaffolds were prepared by micro-molding technique under UV light in the presence of Irgacure2659 (photoinitiator). Their structures were characterized by FTIR analysis. Swelling analysis, mechanical strength test and cell-adhesion test were performed with osteoblast cell line. All results showed that tissue scaffolds with different contents of Mg/Al LDH nanoparticles have great potential in artificial bone tissue production for bone tissue engineering applications.