The use of automated solid phase extraction in the '96 well' format for high throughput bioanalysis using lipid chromatography coupled to tandem mass spectrometry

被引：0
|
作者：
Allanson, JP [1 ]
Biddlecombe, RA [1 ]
Jones, AE [1 ]
Pleasance, S [1 ]
机构：
[1] GLAXO WELLCOME RES & DEV,DIV BIOANAL & DRUG METAB,DEPT INT BIOANAL,BECKENHAM BR3 3BS,KENT,ENGLAND
来源：
RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 1996年 / 10卷 / 07期
关键词：
D O I：
10.1002/(SICI)1097-0231(199605)10:7<811::AID-RCM561>3.0.CO;2-Q
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A high throughput mass spectrometry based method is described for the determination of the 5-HT receptor agonist 311C90, and its desmethyl metabolite, in human plasma, Samples were extracted using the MicroLute(TM) system of solid phase extraction in the '96 well' format, automated by means of a robotic sample processor The extracts were analysed by liquid chromatography tandem mass spectrometry (LC/MS/MS) with thermally assisted electrospray ionization (TurboIonSpray) and selected-reaction monitoring, The LC/MS/MS method offers increased sensitivity, selectivity and speed of analysis over an existing high performance liquid chromotographic method using fluorescence detection.
引用
收藏
页码：811 / 816
页数：6
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