Generation of monodisperse cell-sized microdroplets using a centrifuge-based axisymmetric co-flowing microfluidic device

被引：22
|
作者：
Yamashita, Hitoyoshi [1 ,2 ]
Morita, Masamune [2 ]
Sugiura, Haruka [2 ]
Fujiwara, Kei [3 ]
Onoe, Hiroaki [4 ]
Takinoue, Masahiro [2 ,5 ]
机构：
[1] Tokyo Inst Technol, Sch Biosci & Biotechnol, Midori Ku, Yokohama, Kanagawa 2268502, Japan
[2] Tokyo Inst Technol, Interdisciplinary Grad Sch Sci & Engn, Dept Computat Intelligence & Syst Sci, Midori Ku, Yokohama, Kanagawa 2268502, Japan
[3] Keio Univ, Dept Biosci & Informat, Kohoku Ku, Yokohama, Kanagawa 2238522, Japan
[4] Keio Univ, Dept Mech Engn, Kohoku Ku, Yokohama, Kanagawa 2238522, Japan
[5] Japan Sci & Technol Agcy, PRESTO, Kawaguchi, Saitama 3320012, Japan
来源：
JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2015年 / 119卷 / 04期
基金：
日本科学技术振兴机构; 日本学术振兴会;
关键词：
Monodisperse microdroplets; Microfluidic device; Centrifuge; Plateau-Rayleigh instability; Biochemical reactions; Cell encapsulation; SINGLE-MOLECULE PCR; HIGH-THROUGHPUT; DROPLETS; MICROREACTORS;
D O I：
10.1016/j.jbiosc.2014.09.018
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
We report an easy-to-use generation method of biologically compatible monodisperse water-in-oil microdroplets using a glass-capillary-based microfluidic device in a tabletop mini-centrifuge. This device does not require complicated microfabrication; furthermore, only a small sample volume is required in experiments. Therefore, we believe that this method will assist biochemical and cell-biological experiments. (C) 2014, The Society for Biotechnology, Japan. All rights reserved.
引用
收藏
页码：492 / 495
页数：4
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