Detection of Bacillus Anthracis Using Fluorescence Immunoassay with Quantum Dots Labels

被引：9
|
作者：
Liu Xiao-Hong [1 ,2 ]
Luo Jin-Ping [1 ]
Tian Qing [1 ,2 ]
Liu Chun-Xiu [1 ]
Cai Xin-Xia [1 ,2 ]
机构：
[1] Chinese Acad Sci, Inst Elect, State Key Lab Transducer Technol, Beijing 100190, Peoples R China
[2] Chinese Acad Sci, Grad Sch, Beijing 100190, Peoples R China
来源：
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY | 2011年 / 39卷 / 02期
关键词：
Quantum dots; Bacillus anthracis; Quantitative detection; Specificity; SPORES; ASSAY;
D O I：
10.1016/S1872-2040(10)60414-8
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A method for detecting Bacillus anthracis (B. anthracis) was constructed with indirect immunofluorescence technique using quantum dots (QDs) as fluorescent probes. B. anthracis could be labeled with QDs selectively, that was proved by fluorescence microscope and F4500 spectrofluorometer. The laboratory-made system including biosensor and device was utilized as detection apparatus. A linear relationship of the fluorescence intensity and log total count of B. anthracis was obtained with correlation coefficient of 0. 9554 from 100 CFU/mL to 1 X 10(6) CFU/mL, and a low relative standard deviation of 2. 2%. By comparing the fluorescence intensity of B. anthracis and other bacillus, this method showed good specificity. Because of the simple operation, the detecting time is less (1 h) than traditional methods as well as quantitative detection, this method has potential prospect in monitoring B. anthracis and other biowarfare agents.
引用
收藏
页码：163 / 167
页数：5
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