Multiplex Detection of IgG and IgM to Rift Valley Fever Virus Nucleoprotein, Nonstructural Proteins, and Glycoprotein in Ovine and Bovine

被引：13
|
作者：
Hossain, Mohammad M. [1 ,2 ]
Wilson, William C. [2 ]
Faburay, Bonto [1 ]
Richt, Jurgen [1 ]
McVey, David S. [2 ]
Rowland, Raymond R. [1 ]
机构：
[1] Kansas State Univ, Coll Vet Med, CEEZAD, Dept Diagnost Med Pathobiol, 1800 Denison Ave,N-211 Mosier Hall, Manhattan, KS 66506 USA
[2] ARS, USDA, ABADRU, Ctr Grain & Anim Hlth Res, Manhattan, KS USA
来源：
VECTOR-BORNE AND ZOONOTIC DISEASES | 2016年 / 16卷 / 08期
关键词：
Fluorescence microsphere immunoassay; IgM antibody response; Recombinant RVFV proteins; Rift Valley fever virus; DOMESTIC RUMINANTS; CLIMATE-CHANGE; VACCINATED ANIMALS; POTENTIAL VECTORS; INDIRECT ELISA; CAPTURE ELISA; UNITED-STATES; SAUDI-ARABIA; N-PROTEIN; NSM GENES;
D O I：
10.1089/vbz.2014.1721
中图分类号：
R1 [预防医学、卫生学];
学科分类号：
1004 ; 120402 ;
摘要：
A multiplex fluorescence microsphere immunoassay (FMIA) was used to detect bovine and ovine IgM and IgG antibodies to several Rift Valley fever virus (RVFV) proteins, including the major surface glycoprotein, Gn; the nonstructural proteins, NSs and NSm; and the nucleoprotein, N. Target antigens were assembled into a multiplex and tested in serum samples from infected wild-type RVFV or MP12, a modified live virus vaccine. As expected, the N protein was immunodominant and the best target for early detection of infection. Antibody activity against the other targets was also detected. The experimental results demonstrate the capabilities of FMIA for the detection of antibodies to RVFV structural and nonstructural proteins, which can be applied to future development and validation of diagnostic tests that can be used to differentiate vaccinated from infected animals.
引用
收藏
页码：550 / 557
页数：8
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