Densitometric HPTLC Method for Analysis of Oleanolic Acid in Achyranthes aspera L.

被引：4

作者：

Mehta, Falgun A. ^{[1
]}

Patel, Bharat G. ^{[2
]}

Pandya, Saurabh S. ^{[3
]}

Ahir, Keyur B. ^{[1
]}

机构：

[1] Indukaka Ipcowala Coll Pharm, New Vallabh Vidyanagar 388121, Gujarat, India

[2] AR Coll Pharm, Vallabh Vidyanagar 388120, Gujarat, India

[3] Babariya Coll Pharm, Vadodara 391240, Gujarat, India

来源：

JPC-JOURNAL OF PLANAR CHROMATOGRAPHY-MODERN TLC | 2010年 / 23卷 / 04期

关键词：

High-performance thin-layer chromatography (HPTLC); Oleanolic acid; Triterpenoid saponins; Achyranthes aspera; CHROMATOGRAPHIC DETERMINATION; PLANT SAPONINS;

D O I：

10.1556/JPC.23.2010.4.10

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A densitometric HPTLC method has been established for analysis in alcohol extracts of the seeds and leaves of Achyranthes aspera L. The extracts were separated on aluminum foil-backed silica gel 60 F(254) plates. Detection was by measurement of absorbance at 490 nm, in absorbance/reflectance mode, after derivatization with 10% H(2)SO(4) in 95.5% (v/v) ethanol. The R(F) of oleanolic acid was 0.22. The oleanolic acid content of both extracts was compared statistically and the amount in the seed extract found to be higher (0.970%) than that in the leaf extract (0.666%).

引用

页码：289 / 292

页数：4

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