Study on pharmaco kinetic of ofloxacin by paper substrate-room temperature phosphorimetry

The ofloxacin concentration in urine was determined by using paper substrate room temperature phosphorimetry (PS-RTP). The result shows that the recovery of standard samples added to urine is 99.1%, relative standard deviation is less than 1.7%. The excretion rate of ofloxacin in the urine of three healthy volunteers' was assayed by PS-RTP. The ofloxacin is excreted through kidneys in its original form, after taking medicine, the excretion rate of ofloxacin within 24 hours is 77.7%, the highest concentration of ofloxacin will appear between 2.5 and 4 hours. The method is quick and simple. The experimental results are satisfactory.