Genetic polymorphism in IGF-1 gene in four sheep and goat breeds and its association with biometrical traits in migratory Gaddi goat breed of western Himalayan state of Himachal Pradesh, India

被引:8
|
作者
Sankhyan, Varun [1 ]
Thakur, Y. P. [2 ]
Dogra, P. K. [1 ]
机构
[1] CSK Himachal Pradesh Krishi Vishvavidyalaya, Coll Vet & Anim Husb, Dept Anim Genet & Breeding, Palampur 176062, Himachal Prades, India
[2] CSKHPKV Palampur, COVAS, Dept Livestock Prod Management, Palampur 176062, Himachal Prades, India
关键词
Genetic polymorphism; IGF-1; gene; Migratory system; Small ruminants; SINGLE NUCLEOTIDE POLYMORPHISM; GROWTH TRAITS; I GENE; BODY-SIZE; WEIGHT;
D O I
10.18805/ijar.B-3795
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
Sheep and goat production is a predominant livestock activity in harsh climatic regions of the country particularly in hilly areas. The goat and sheep population in Himachal Pradesh is 1.12 and 0.81 million respectively. Gaddi and Rampur-Bushair are the two indigenous sheep breeds of the state well adapted to the prevalent migratory production system. The somatotrophic axis (GH/IGF-I axis) is considered as the key in postnatal growth and metabolism in different mammals including farm animals. The technique of PCR-RFLP was used to analyze polymorphism of IGF-1 gene in sheep and goat breeds of Himachal Pradesh and to study association with body measurements in Gaddi goats. For polymorphism studies, genomic DNA was isolated from 63, 68, 197 and 73 animals belonging to Gaddi sheep, Rampur-Bushair sheep, Gaddi goat and Chegu goats, respectively. PCR amplification of IGF-1 yielded 363 bp amplicon. The PCR-RFLP digestion of amplified products with Haelll revealed three fragments of 363 bp, 264 bp and 99 bp after digestion. Allele frequencies for A allele were 0.66 0.59, 0.65 and 0.70 and for B allele were 0.34, 0.41, 0.35 and 0.20 in Gaddi sheep, Rampur-Bushair sheep, Gaddi goat and Chegu goat, respectively. The frequency of AA, AB and BB genotypes ranged from 0.38 to 0.55, 0.30 to 0.42 and 0.14 to 0.19 in different breeds studied. The N-e, H-obs and H-exp ranged from 1.80 to 1.99, 0.30 to 0.43, 0.42 to 0.48 and 0.33 to 0.37, respectively in different populations. The PIC revealed median level of polymorphism in studied breeds. Alignment of sequences also confirmed presence of C/G substitution at 264 bp. Nucleotide variability was found at 17 places between different breeds. Genetic variability was detected among all four breeds studied, however no significant association could be detected in Gaddi goats.
引用
收藏
页码:508 / 512
页数:5
相关论文
共 3 条
  • [1] Polymorphism of the VEGF gene and its association with growth traits in four goat breeds
    Jin, Q. J.
    Fang, X. T.
    Zhang, C. L.
    Yang, L.
    Sun, J. J.
    Chen, D. X.
    Shi, X. Y.
    Du, Y.
    Lan, X. Y.
    Chen, H.
    SOUTH AFRICAN JOURNAL OF ANIMAL SCIENCE, 2010, 40 (01) : 33 - 40
  • [2] Association of kisspeptin gene (KISS1) with litter size in migratory Gaddi goats in western Himalayan state of Himachal Pradesh
    Sankhyan, Varun
    Thakur, Y. P.
    Dogra, Pardeep Kumar
    INDIAN JOURNAL OF ANIMAL SCIENCES, 2019, 89 (12): : 1352 - 1355
  • [3] Polymorphism of the Insulin-like growth factor 1 receptor gene (IGF1R/e10/MspI and IGF1R/e16/RsaI) in four dairy breeds and its association with milk traits
    Szewczuk, Malgorzata
    LIVESTOCK SCIENCE, 2015, 181 : 43 - 50