A simple and low cost structured illumination microscopy using a pico-projector

被引：0
|
作者：
Ozgurun, Baturay [1 ,2 ]
机构：
[1] Sabanci Univ, Elect Engn, Istanbul, Turkey
[2] Istanbul Medipol Univ, Sch Engn & Nat Sci, Istanbul, Turkey
来源：
SINGLE MOLECULE SPECTROSCOPY AND SUPERRESOLUTION IMAGING XI | 2018年 / 10500卷
关键词：
Super resolution; microscopy; SIM; pico-projector; structured illumination; RESOLUTION; LIMIT;
D O I：
10.1117/12.2286790
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
Here, development of a low-cost structured illumination microscopy (SIM) based on a pico-projector is presented. The pico-projector consists of independent red, green and blue LEDs that remove need for an external illumination source. Moreover, display element of the pico-projector serves as a pattern generating spatial light modulator. A simple lens group is employed to couple light from the projector to an epi-illumination port of a commercial microscope system. 2D sub SIM images are acquired and synthesized to surpass the diffraction limit using 40x (0.75 NA) objective. Resolution of the reconstructed SIM images is verified with a dye-and-object object and a fixed cell sample.
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页数：6
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