Rapid Yeast Cell Viability Analysis by Using a Portable Microscope Based on the Fiber Optic Array and Simple Image Processing

被引：2

作者：

Wang, Weiming ^{[1
,2
]}

Liu, Hang ^{[1
,2
]}

Yu, Yan ^{[1
,2
]}

Cong, Fengyu ^{[1
]}

Yu, Jun ^{[1
,2
]}

机构：

[1] Dalian Univ Technol, Fac Elect Informat & Elect Engn, 2 Linggong Rd, Dalian 116024, Peoples R China

[2] Dalian Univ Technol, Key Lab Integrated Circuit & Biomed Elect Syst Li, Dalian 116023, Peoples R China

来源：

SENSORS | 2020年 / 20卷 / 07期

关键词：

FOA; lens; yeast activity; yeast concentration; portable microscope; SCHISTOSOMA; DIAGNOSIS;

D O I：

10.3390/s20072092

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A fiber optic array (FOA) can be used as an alternative or a supplement to the lens in a microscope due to its large magnification, high coupling efficiency and extremely low distortion. Based on our previous research, this paper first demonstrated the resolution and field-of-view (FOV) of the microscope based on the FOA. To further validate the FOA microscope's imaging capability, yeast activity and concentration were investigated by simple image processing. The results showed that the percentages of live and dead yeast cells correctly identified were 92.1% and 84.8%, except for the clusters, which agreed well with the manual counting methods. Then, the performances of the portable microscopes based on the FOA and lens were compared and the factors that affect the FOA microscope imaging performance were analyzed.

引用

页数：10

共 14 条

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[8] An automated method for in vitro anticancer drug efficacy monitoring based on cell viability measurement using a portable photodiode array chip
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[10] In vitro discrimination and classification of Microbial Flora of Poultry using two dispersive Raman spectrometers (microscope and Portable Fiber-Optic systems) in tandem with chemometric analysis
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