Delayed Measurement of Eosin-5-Maleimide Binding May Affect the Test Results of Highly Hemolyzed Samples In Vivo and In VitroA Case Study

被引：3

作者：

Ciepiela, Olga ^{[1
]}

Adamowicz-Salach, Anna ^{[2
]}

Zdziechowicz, Izabela ^{[2
]}

Kotula, Iwona ^{[1
]}

机构：

[1] Med Univ Warsaw, Dept Lab Diagnost & Clin Immunol Dev Age, Zwirki & Wigury 63a St, PL-02091 Warsaw, Poland

[2] Med Univ Warsaw, Dept Pediat Haematol & Oncol, Warsaw, Poland

来源：

JOURNAL OF PEDIATRIC HEMATOLOGY ONCOLOGY | 2016年 / 38卷 / 08期

关键词：

eosin-5-maleimide; hemolysis; hereditary spherocytosis; systemic lupus erythematosus; HEREDITARY SPHEROCYTOSIS; SCREENING-TEST; DIAGNOSIS; BAND-3;

D O I：

10.1097/MPH.0000000000000652

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Diagnosis of hereditary spherocytosis (HS) is based on clinical evaluation and eosin-5-maleimide (EMA) test. A decrease in EMA fluorescence compared with healthy individuals is typical for HS and serves as a basis for HS diagnosis. Sensitivity and specificity of the test is high and false-positive results rarely occur. Studies have shown that anticoagulated blood sample when stored at 4 degrees C for 7 days do not affect the test results. This case study is about an autoimmune hemolytic anemia patient who showed a primary positive result for EMA test (decrease in EMA fluorescence47% compared with 100% for samples of healthy individual), when the test was performed in the sample stored for 48 hours after venipuncture and before staining. An irrelevant decrease (92.5% compared with 100% for samples of healthy individual) was found when freshly collected sample was analyzed. On the basis of the results obtained, it is recommended that EMA staining should be performed on the same day of blood collection for patients with significant hemolysis.

引用

页码：E303 / E306

页数：4