Label-free microscopy: A non-invasive new tool to assess gametes and embryo quality

被引：6
|
作者：
Rubessa, Marcello [1 ]
Wheeler, Matthew B. [1 ,2 ,3 ]
机构：
[1] Dept Anim Sci, Urbana, IL USA
[2] Beckman Inst Adv Sci & Technol, Urbana, IL USA
[3] Univ Illinois, Dept Bioengn, Urbana, IL USA
来源：
THERIOGENOLOGY | 2020年 / 150卷
关键词：
Microscopy; Label-free microscopy; Sperm; Oocytes; Embryos; TIME-LAPSE; SPERM; INTEROBSERVER; SELECTION; GROWTH; OOCYTE;
D O I：
10.1016/j.theriogenology.2020.01.065
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
In PubMed, it is possible to find more than 40,000 papers on embryo evaluation in various species. However, there is no consensus or gold standard method on how to assess their developmental potential. In assisted reproduction the evaluation "problem" is not only limited to embryos but involves the gametes as well. This manuscript provides an overview of some possible applications of label-free microscopy, in particular we describe the potential of the holographic microscopy in the IVF lab. We describe the positive aspects of several currently available microscopy label-free systems. In conclusion, we believe that a next generation of microscopy able to give objective markers for gamete and embryo quality is around the corner. (C) 2020 Elsevier Inc. All rights reserved.
引用
收藏
页码：241 / 246
页数：6
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