Simultaneous Analysis of 2-and 4-Methylimidazole in Caramel Color and Soft Drinks Using IC-PAD with Post-Column Addition of Hydroxide

被引：12

作者：

Xu, Xian-Bing ^{[1
]}

Liu, Ding-Bo ^{[1
]}

Zhao, Yi ^{[1
]}

Yu, Shu-Juan ^{[1
,2
]}

Zhao, Zhen-Gang ^{[1
]}

机构：

[1] S China Univ Technol, Coll Light Ind & Food Sci, Guangzhou 510640, Guangdong, Peoples R China

[2] Guangdong Prov Key Lab Green Proc Nat Prod & Prod, Guangzhou 510640, Guangdong, Peoples R China

来源：

FOOD ANALYTICAL METHODS | 2015年 / 8卷 / 02期

基金：

中国国家自然科学基金; 美国国家科学基金会;

关键词：

4-Methylimidazole; 2-Methylimidazole; HPCEC-PAD; Solid-phase extraction; Soft drink; Caramel color; MASS-SPECTROMETRIC QUANTIFICATION; ION-PAIR EXTRACTION; CARCINOGENIC 4(5)-METHYLIMIDAZOLE; BEVERAGES; COFFEE; FOODS;

D O I：

10.1007/s12161-014-9917-4

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

High-performance cation exchange chromatography with pulsed integrated amperometric electrochemical detector (HPCEC-PAD) with post-column addition of hydroxide was first developed for simultaneous analysis of 4-methylimidazole (4-MeI) and 2-methylimidazole (2-MeI) in caramel color and soft drinks after solid-phase extraction (SPE). A CS12A cation exchange column was used to separate the targeted analytes with a perfect resolution (2.70). This method demonstrated low limit of quantification (0.05-10 mg/L) and excellent linearity with correlation of determination (R (2) > 0.999 for 2-MeI and 0.997 for 4-MeI). Low concentrations of 4-MeI were found in five soft drinks, whereas those in caramel color were generally high. Additionally, the suggested method showed higher resolution and sensitivity comparing with previous reported methods for 4-MeI and 2-MeI analyses.

引用

页码：467 / 473

页数：7