Photochemically amplified detection of molecular recognition events: an ultra-sensitive fluorescence turn-off binding assay

被引：14
|
作者：
Gustafson, Tiffany P. [1 ]
Metzel, Greg A. [1 ]
Kutateladze, Andrei G. [1 ]
机构：
[1] Univ Denver, Dept Chem & Biochem, Denver, CO 80208 USA
来源：
ORGANIC & BIOMOLECULAR CHEMISTRY | 2011年 / 9卷 / 13期
关键词：
BIO-BARCODE ASSAY; SIGNAL AMPLIFICATION; PROTEIN;
D O I：
10.1039/c1ob05289f
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
Amplified fluorescence quenching methodology based on massive autocatalytic photo-unmasking of a dual function sensitizer-quencher is developed and adopted for photoassisted ultra-sensitive detection of molecular recognition events. The resulting binding assay, based on a molecular recognition-triggered photo-amplified cascade with concomitant decrease of fluorescence is validated with the biotin-avidin pair, achieving attomolar detection.
引用
收藏
页码：4752 / 4755
页数：4
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