Single molecule biochemistry using optical tweezers

被引：15
|
作者：
Mehta, AD [1 ]
Pullen, KA [1 ]
Spudich, JA [1 ]
机构：
[1] Stanford Univ, Dept Biochem, Ctr Med, Stanford, CA 94305 USA
来源：
FEBS LETTERS | 1998年 / 430卷 / 1-2期
关键词：
optical trap; single molecule; myosin; kinesin; titin;
D O I：
10.1016/S0014-5793(98)00594-8
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The use of optical trapping to create extremely compliant mechanical probes has ushered in a new field of biological inquiry, the mechanical and kinetic study of proteins at the single molecule level, This review focuses on three examples of such study and includes methods of extracting parameters of interest from the raw data such experiments generate. (C) 1998 Federation of European Biochemical Societies.
引用
收藏
页码：23 / 27
页数：5
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