Emergence and spread of NADC34-like PRRSV in Southwest China

In recent years, NADC34-like PRRSV had a strong impact on the pig industry in the United States and Peru; it was also detected in northeastern China in 2017. In this study, we conducted a retrospective survey of NADC34-like PRRSV in Southwest China from 2016 to 2020. Five NADC34-like PRRSV strains were detected in samples and their whole genomes were sequenced, designated as CHSCMY-22019, CHSCYB-32020, CHSCMS-42020 and CHSCLS-22020. This is the first discovery and report of an NADC34-like PRRSV strain in Southwest China. Phylogenetic tree analysis based on the whole genome showed that the five NADC34-like PRRSV strains belonged to sub-lineage 1.5 of PRRV-2. They had 100 aa deletions in the Nsp2 hypervariable region of VR2332, located at 329-428 aa, similar to the US isolate IA/2014/NADC34. Recombination analysis showed that CHSCCD-42020 strain was the recombinant strain of QYYZ strain and IA/2014/NADC34 strain in China. The emergence of NADC34-like PRRSV strains in Southwest China indicates a potential threat to PRRS prevention and control in pigs. This study improves our understanding of the epidemic status and genetic variation of NADC34-like PRRSV strains in China.