Sensitivity of Francisella tularensis to ultrapure water and deoxycholate: Implications for bacterial intracellular growth assay in macrophages

被引：7
|
作者：
Chalabaev, Sabina
Anderson, Christine A.
Onderdonk, Andrew B.
Kasper, Dennis L. [1 ]
机构：
[1] Harvard Univ, Sch Med, Dept Microbiol & Mol Genet, Boston, MA 02115 USA
来源：
JOURNAL OF MICROBIOLOGICAL METHODS | 2011年 / 85卷 / 03期
关键词：
Francisella tularensis; Intracellular growth; Lysis; SCHU S4; VIRULENCE; LVS;
D O I：
10.1016/j.mimet.2011.03.006
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The ability of Francisella tularensis to replicate in macrophages is critical for its pathogenesis, therefore intracellular growth assays are important tools for assessing virulence. We show that two lysis solutions commonly used in these assays, deionized water and deoxycholate in PBS, lead to highly inaccurate measurements of intracellular bacterial survival. (C) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码：230 / 232
页数：3
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