Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry in clinical chemistry

被引:160
|
作者
Marvin, LF
Roberts, MA
Fay, LB
机构
[1] Nestec Ltd, Nestle Res Ctr, CH-1000 Lausanne 26, Switzerland
[2] Mail Zone IIT, Nestle Purina Pet Care, St Louis, MO 63164 USA
关键词
MALDI-MS; proteins; markers; microbiology; genotyping;
D O I
10.1016/j.cccn.2003.08.008
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-Tof-MS) has recently become a Popular and versatile method to analyze macromolecules from biological origin. In this paper, we will review the application of MALDI-Tof-MS in clinical chemistry and biology. MALDI-Tof-MS is used in clinical chemistry, e.g. disease markers call be identified with MALDI-MS analysis in combination with 1-D and 2-D gel electrophoresis separations thanks to either peptide mass fingerprinting (PMF) or peptide sequence tag (PST) followed by data base searching. In microbiology, MALDI-Tof-MS is employed to analyze specific peptides or proteins directly desorbed from intact viruses, bacteria and spores. The capability to register biomarker ions in a broad m/z range, which are unique and representative for individual microorganisms, forms the basis of taxonomic identification of bacteria by MALDI-Tof-MS. Moreover, this technique call be applied to study either the resistance of bacteria to antibiotics or the antimicrobial compounds secreted by other bacterial species. More recently, the method was also successfully applied to DNA sequencing (genotyping) as well as screening for mutations. High-throughput genotyping of single-nucleotide polymorphisms has the potential to become a routine method for both laboratory and clinical applications. Moreover, posttranscriptional modifications of RNA can be analyzed by MALDI using nucleotide-specific RNAses combined with further fragmentation by post source decay (PSD). (C) 2003 Elsevier B.V. All rights reserved.
引用
收藏
页码:11 / 21
页数:11
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