Improved adenylate cyclase activity via affinity immobilization onto co-modified GO with bio-inspired adhesive and PEI

被引：18

作者：

Liu, Tongle ^{[1
]}

Rao, Yuan ^{[1
,2
]}

Zhou, Wenfeng ^{[1
]}

Zhuang, Wei ^{[1
,3
]}

Ge, Lei ^{[4
,5
]}

Lin, Rijia ^{[5
]}

Zhang, Wenxia ^{[2
]}

Wu, Jinglan ^{[1
,3
]}

Zhu, Chenjie ^{[1
,3
]}

Ying, Hanjie ^{[1
,3
]}

机构：

[1] Nanjing Tech Univ, Coll Biotechnol & Pharmaceut Engn, Natl Engn Tech Res Ctr Biotechnol, State Key Lab Mat Oriented Chem Engn, 30 Puzhu South Rd, Nanjing 211816, Peoples R China

[2] Zhengzhou Univ, Sch Chem Engn, Zhengzhou 450001, Peoples R China

[3] Nanjing Tech Univ, Synerget Innovat Ctr Adv Mat, 30 Puzhu South Rd, Nanjing 211816, Peoples R China

[4] Univ Southern Queensland, Ctr Future Mat, Brisbane, Qld 4300, Australia

[5] Univ Queensland, Sch Chem Engn, St Lucia, Qld 4072, Australia

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 2021年 / 205卷

基金：

中国国家自然科学基金;

关键词：

Graphene oxide; Adenylate cyclase; Polydopamine; Polyethyleneimine; Affinity; GRAPHENE OXIDE; MAGNETIC GRAPHENE; AMINO-ACIDS; POLYETHYLENEIMINE; ENHANCEMENT; EFFICIENCY; NANOSHEETS; CHEMISTRY; LIPASE; GROWTH;

D O I：

10.1016/j.colsurfb.2021.111888

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Adenylate cyclase (AC) can efficiently catalyze the conversion of adenosine triphosphate (ATP) to cyclic adenosine-3 ', 5 '-monophosphate (cAMP). However, AC directly immobilized on substrate is not desirable due to enzyme inactivation. Herein, bio-inspired adhesive of polydopamine and polyethyleneimine (PDA/PEI) was used as flexible chains to graft on graphene oxide (GO), and the AC was directionally immobilized through affinity between metal ions and his-tags of AC. The properties of modified GO and the activity of immobilized AC were studied in detail. PDA/PEI layers have been proved to improve the amino density of GO surface for affinity groups decoration and adjust the interaction between AC and support. And modified GO by this novel method contributes to subsequent grafting and immobilization of AC by affinity. AC immobilized on modified GO exhibited high activity recovery with about 90 % of free AC, while enzyme immobilized on unmodified GO has been inactivated. This study offers a versatile approach for support modification and enzyme oriented immobilization. PDA/PEI functionalized GO can be used as a promising carrier to immobilize other his-tagged enzymes.

引用

页数：10