Visualization of transfection of hepatocytes by galactosylated chitosan-graft-poly(ethylene glycol)/DNA complexes by confocal laser scanning microscopy

被引：37
|
作者：
Park, IK
Kim, TH
Kim, SI
Park, YH
Kim, WJ
Akaike, T
Cho, CS [1 ]
机构：
[1] Seoul Natl Univ, Sch Agr Biotechnol, Suwon 441744, South Korea
[2] Seoul Natl Univ, Sch Biol Res & Mat Engn, Suwon 441744, South Korea
[3] Tokyo Inst Technol, Dept Biomol Engn, Yokohama, Kanagawa 2268501, Japan
来源：
INTERNATIONAL JOURNAL OF PHARMACEUTICS | 2003年 / 257卷 / 1-2期
关键词：
chitosan; galactose; PEG; nuclear trafficking; Confocal laser scanning microscopy; hepatocyte;
D O I：
10.1016/S0378-5173(03)00133-9
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
Dual-labeled galactosylated chitosan-graft-poly(ethylene glycol) (PEG) (GCP)/DNA complexes were prepared and their hepatocyte-specific delivery and cellular distribution were investigated by confocal laser scanning microscopy (CLSM). The complexes were transfected into hepatocyte through specific interaction of galactose moiety of the GCP and asialoglycoprotein receptors (ASGPR) of the hepatocytes. The GCP/DNA complexes taken up by the hepatocytes were rapidly released into the cytoplasm, but nuclear trafficking of the released complexes was slow and rate-limiting process. The more efficient transfection of the complex occurred in the human-derived HepG2 cells than in primary hepatocytes. (C) 2003 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：103 / 110
页数：8
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