Roles of SWI/SNF and HATs throughout the dynamic transcription of a yeast glucose-repressible gene

被引:58
|
作者
Geng, FQ [1 ]
Laurent, BC [1 ]
机构
[1] SUNY Downstate Med Ctr, Morse Inst Mol Genet, Dept Microbiol & Immunol, Mol & Cellular Biol Program, Brooklyn, NY 11203 USA
来源
EMBO JOURNAL | 2004年 / 23卷 / 01期
关键词
biphasic; Esa1; Gcn5; stress; SWI/SNF;
D O I
10.1038/sj.emboj.7600035
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Eucaryotic gene expression requires chromatin-remodeling activities. We show by time-course studies that transcriptional induction of the yeast glucose-regulated SUC2 gene is rapid and shows a striking biphasic pattern, the first phase of which is partly mediated by the general stress transcription factors Msn2p/Msn4p. The SWI/ SNF ATP-dependent chromatin-remodeling complex associates with the promoter in a similar biphasic manner and is essential for both phases of transcription. Two different histone acetyltransferases, Gcn5p and Esa1p, enhance the binding of SWI/ SNF to the promoter during early transcription and are required for optimal SUC2 induction. Gcn5p is recruited to SUC2 simultaneously with SWI/ SNF, whereas Esa1p associates constitutively with the promoter. This study reveals an unusual transcription pattern of a metabolic gene and suggests a novel strategy by which distinct chromatin remodelers cooperate for the dynamic activation of transcription.
引用
收藏
页码:127 / 137
页数:11
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