Schisantherin A induces cell apoptosis through ROS/JNK signaling pathway in human gastric cancer cells

被引:42
|
作者
Wang, Zishu [1 ]
Yu, Kaikai [2 ]
Hu, Yudong [2 ]
Su, Fang [1 ]
Gao, Zhenyuan [1 ]
Hu, Ting [1 ]
Yang, Yang [1 ]
Cao, Xiangliao [1 ]
Qian, Feng [1 ,2 ,3 ]
机构
[1] Bengbu Med Coll, Affiliated Hosp 1, Dept Med Oncol, Anhui Prov Key Lab Translat Canc Res, Bengbu 233004, Anhui, Peoples R China
[2] Shanghai Jiao Tong Univ, Engn Res Ctr Cell & Therapeut Antibody, Sch Pharm, Minist Educ, Shanghai 200240, Peoples R China
[3] Xuzhou Med Univ, Jiangsu Ctr Collaborat & Innovat Canc Biotherapy, Canc Inst, Xuzhou 221004, Jiangsu, Peoples R China
基金
中国国家自然科学基金;
关键词
Schisantherin A; Gastric cancer; ROS; JNK; Apoptosis; NF-KAPPA-B; CYCLE ARREST; HEPATOCELLULAR-CARCINOMA; INHIBITION; DEATH; ACTIVATION; INDUCTION; PROTECTS; MICE; JNK;
D O I
10.1016/j.bcp.2019.113673
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Gastric cancer is one of the most lethal cancers with unmet clinical treatment and low 5-year survival rate. Schisantherin A is a major compound derived from Fructusschisandrae while its anti-tumor role remains nearly unknown. Here, we reported that schisantherin A had an anti-proliferation effect on gastric cancer cell lines MKN45 and SGC-7901. Schisantherin A induced cell cycle arrest at G2/M phase and cell apoptosis, and inhibited cell migration in gastric cancer MKN45 and SGC7901 cells. Meanwhile, upregulation of cleaved caspase-9, cleaved caspase-3 and cleaved PARP were accompanied with the loss of mitochondrial membrane potential (MMP). Moreover, schisantherin A induced ROS-dependent JNK phosphorylation with higher ROS production. The JNK inhibitor and ROS scavenger NAC rescued the cell apoptosis and cycle inhibition elicited by schisantherin A. Furthermore, the expression level of antioxidant factor Nrf2 was suppressed by schisantherin A. These findings suggest that schisantherin A possesses an anti-tumor activity via activation of ROS/JNK with Nrf2 inhibition, indicating that schisantherin A is a promising chemotherapeutic candidate for gastric cancer.
引用
收藏
页数:12
相关论文
共 50 条
  • [1] Vernodalin induces apoptosis through the activation of ROS/JNK pathway in human colon cancer cells
    Mohebali, Nooshin
    Pandurangan, Ashok Kumar
    Mustafa, Mohd Rais
    Anandasadagopan, Suresh Kumar
    Alagumuthu, Tamilselvi
    JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY, 2020, 34 (12)
  • [2] Metformin induces cell cycle arrest, apoptosis and autophagy through ROS/JNK signaling pathway in human osteosarcoma
    Li, Bo
    Zhou, Pingting
    Xu, Kehan
    Chen, Tianrui
    Jiao, Jian
    Wei, Haifeng
    Yang, Xinghai
    Xu, Wei
    Wan, Wei
    Xiao, Jianru
    INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES, 2020, 16 (01): : 74 - 84
  • [3] Loperamide induces protective autophagy and apoptosis through the ROS/ JNK signaling pathway in bladder cancer
    Wu, Jianjian
    Guo, Qiang
    Li, Juntao
    Yuan, Hao
    Xiao, Chutian
    Qiu, Jianguang
    Wu, Qiong
    Wang, Dejuan
    BIOCHEMICAL PHARMACOLOGY, 2023, 218
  • [4] Ziyuglycoside II induces cell cycle arrest and apoptosis through activation of ROS/JNK pathway in human breast cancer cells
    Zhu, Xue
    Wang, Ke
    Zhang, Kai
    Zhu, Ling
    Zhou, Fanfan
    TOXICOLOGY LETTERS, 2014, 227 (01) : 65 - 73
  • [5] Neohesperidin Induces Cell Cycle Arrest, Apoptosis, and Autophagy via the ROS/JNK Signaling Pathway in Human Osteosarcoma Cells
    Wang, Shubin
    Li, Zongguang
    Liu, Wei
    Wei, Guojun
    Yu, Naichun
    Ji, Guangrong
    AMERICAN JOURNAL OF CHINESE MEDICINE, 2021, 49 (05): : 1251 - 1274
  • [6] Hispolon induces apoptosis in human gastric cancer cells through a ROS-mediated mitochondrial pathway
    Chen, Wei
    Zhao, Zhao
    Li, Ling
    Wu, Bin
    Chen, Shi-fei
    Zhou, Hong
    Wang, Yong
    Li, Yong-Quan
    FREE RADICAL BIOLOGY AND MEDICINE, 2008, 45 (01) : 60 - 72
  • [7] Nonylphenol Induces Apoptosis through ROS/JNK Signaling in a Spermatogonia Cell Line
    Park, Hyun-Jung
    Lee, Ran
    Yoo, Hyunjin
    Hong, Kwonho
    Song, Hyuk
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2021, 22 (01) : 1 - 14
  • [8] XEDAR inhibits the proliferation and induces apoptosis of gastric cancer cells by regulating JNK signaling pathway
    Yang, Lihong
    Huang, Xiaojun
    Wang, Wei
    Jiang, Tao
    Ding, Feifei
    BIOSCIENCE REPORTS, 2019, 39
  • [9] Andrographolide induces apoptosis in human osteosarcoma cells via the ROS/JNK pathway
    Wang, Shengdong
    Li, Hengyuan
    Chen, Shi
    Wang, Zenan
    Yao, Yuhong
    Chen, Tao
    Ye, Zhaoming
    Lin, Peng
    INTERNATIONAL JOURNAL OF ONCOLOGY, 2020, 56 (06) : 1417 - 1428
  • [10] RETRACTION: XEDAR inhibits the proliferation and induces apoptosis of gastric cancer cells by regulating JNK signaling pathway
    Yang, Lihong
    Huang, Xiaojun
    Wang, Wei
    Jiang, Tao
    Ding, Feifei
    BIOSCIENCE REPORTS, 2024, 44 (08)