The importance of applying confirmatory tests to detect infectious salmon anaemia virus (ISAV) in environmental samples by RT-PCR

被引：0
|
作者：
Gregory, A. [1 ]
Munro, L. A. [1 ]
Ostergard, P. S. [1 ]
Raynard, R. S. [1 ]
机构：
[1] Marine Lab, Fisheries Res Serv, Aberdeen AB11 9DB, Scotland
来源：
BULLETIN OF THE EUROPEAN ASSOCIATION OF FISH PATHOLOGISTS | 2007年 / 27卷 / 03期
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D O I：
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中图分类号：
S9 [水产、渔业];
学科分类号：
0908 ;
摘要：
When analysing seawater samples for ISAV by RT-PCR, a non-specific PCR product approximately the size expected for ISAV was obtained. DNA sequence analysis and southern hybridisation established it was not ISAV. A confirmatory test should be used with RT-PCR for ISAV detection in environmental samples to avoid misinterpretation of results.
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页码：123 / 127
页数：5
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