Occurrence of conjugative IncF-type plasmids harboring the blaCTX-M-15 gene in Enterobacteriaceae isolates from newborns in Tunisia

被引：11
|
作者：
Lahlaoui, Hella [1 ]
De Luca, Filomena [2 ]
Maradel, Simona [2 ]
Ben-Haj-Khalifa, Anis [3 ]
Ben Hamouda, Hechmi [3 ]
Kheder, Mohamed [3 ]
Ben Moussa, Mohamed [1 ]
Rossillini, Gian-Maria [2 ]
Docquier, Jean-Denis [2 ]
机构：
[1] Mil Hosp Tunis, Microbiol Lab, Monfleury, Tunisia
[2] Univ Siena, Dept Med Biotechnol, I-53100 Siena, Italy
[3] Hosp Tahar Sfar, Microbiol Lab, Mahdia, Tunisia
来源：
PEDIATRIC RESEARCH | 2015年 / 77卷 / 01期
关键词：
SPECTRUM BETA-LACTAMASES; CTX-M-TYPE; COMPLETE NUCLEOTIDE-SEQUENCE; ESCHERICHIA-COLI; KLEBSIELLA-PNEUMONIAE; ADDICTION SYSTEMS; DISSEMINATION; CTX-M-15; STRAINS; PREVALENCE;
D O I：
10.1038/pr.2014.153
中图分类号：
R72 [儿科学];
学科分类号：
100202 ;
摘要：
BACKGROUND: CTX-M-15 is the dominant type of extended-spectrum beta-lactamase in clinical isolates. This enzyme constitutes the most widespread enzymes in Tunisia. In this study, we were interested to understand the causes of the evolutionary success of CTX-M-15 in a Tunisian university hospital. METHODS: A total of of 72 cefotaxime-resistant Enterobacteriaceae were isolated from newborn patients at the hospital Taher sfar Mandia in Tunisia and characterized their genetic support by means of molecular techniques. RESULTS: Isolates were clustered into various clonal groups, although most isolates belonged to sequence types ST39 (Klebsiella pneumoniae) and ST131 (Escherichia coli). F replicons (FIA, FIB, and Fit) were the most frequently detected replicon types in our collection (91.66%). CONCLUSION: This is the first report of QnrB- and CTX-M-15-encoding large IncF-type conjugative plasmids in Tunisia.
引用
收藏
页码：107 / 110
页数：4
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