A one-step PCR-based method for specific identification of 10 common lactic acid bacteria and Bifidobacterium in fermented milk

被引：12
|
作者：
Lu, Wenwei [1 ]
Kong, Wentao [1 ]
Yang, Pu [1 ]
Kong, Jian [1 ]
机构：
[1] Shandong Univ, State Key Lab Microbial Technol, Jinan 250100, Peoples R China
来源：
INTERNATIONAL DAIRY JOURNAL | 2015年 / 41卷
基金：
中国国家自然科学基金;
关键词：
16S-23S RIBOSOMAL-RNA; RAPID IDENTIFICATION; LACTOBACILLUS; LEUCONOSTOC; PRIMERS;
D O I：
10.1016/j.idairyj.2014.08.020
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
A one-step PCR-based method was constructed for the rapid detection of 10 common lactic acid bacteria (LAB) and Bifidobacterium. Ten primer pairs were designed based on the sequences that specify the 16S rRNA genes of Lactococcus lactis subsp. lactis, Lactococcus lactis subsp. cremoris, Streptococcus thermophilus, Lactobacillus delbrueckii subsp. bulgaricus, Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus fermentum, Lactobacillus helveticus, Bifidobacterium and Leuconostoc. The primers showed high specificity for target species. The detection limit was 10(4)-10(5) cfu mL(-1) for Lb. delbrueckii and 10(3)-10(4) cfu mL(-1) for the others. This method was applied to identify the species mentioned above, in situ, from 8 samples of traditional fermented milk, and monitor the constitution of the species in commercial dairy yoghurt. The results were consistent with the results of 16S rRNA sequencing, suggesting that the method was reliable for the rapid identification of LAB and Bifidobacterium used in fermented milk. (C) 2014 Elsevier Ltd. All rights reserved.
引用
收藏
页码：7 / 12
页数：6
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