Affibody molecule-mediated depletion of HSA and IgG using different buffer compositions: a 15 min protocol for parallel processing of 1-48 samples

被引：9

作者：

Eriksson, Cecilia ^{[1
]}

Schwenk, Jochen M. ^{[1
]}

Sjoberg, Anna ^{[1
]}

Hober, Sophia ^{[1
]}

机构：

[1] Royal Inst Technol, AlbaNova Univ Ctr, Dept Biotechnol, SE-10691 Stockholm, Sweden

来源：

BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY | 2010年 / 56卷

基金：

瑞典研究理事会;

关键词：

Affibody molecule; human serum albumin (HSA); IgG; protein depletion; high throughput analysis; sample preparation; serum or plasma analysis; HUMAN SERUM; ABUNDANT PROTEINS; HUMAN-PLASMA; MASS-SPECTROMETRY; FRACTIONATION; CHROMATOGRAPHY; MICROARRAYS; VALIDATION; PROTEOMICS; ALBUMIN;

D O I：

10.1042/BA20100041

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

High-abundant plasma proteins pose a challenge in a large number of proteomics-based technologies. Depletion of these high-abundant proteins has proven to be a fruitful strategy to circumvent masking of lower-abundant proteins that could serve as valuable biomarker candidates. However, current strategies often do not meet the throughput requirements of large-scale proteomic studies. In the present paper, a flexible and parallelized method for the depletion of high-abundant proteins is described, allowing the removal of the two most abundant proteins from 48 blood-derived samples in less than 15 min using Affibody molecules as affinity ligands. A sample-processing platform like this should be suitable for a number of proteomics technologies; its flexibility in buffer composition allows for different types of downstream applications.

引用

页码：49 / 57

页数：9