Rapid and efficient detection of Mycobacterium tuberculosis in respiratory and non-respiratory samples

被引：66
|
作者：
Malbruny, B. [1 ]
Le Marrec, G. [1 ]
Courageux, K. [1 ]
Leclercq, R. [1 ]
Cattoir, V. [1 ]
机构：
[1] Ctr Hosp Univ Cote de Nacre, Microbiol Serv, Caen, France
来源：
INTERNATIONAL JOURNAL OF TUBERCULOSIS AND LUNG DISEASE | 2011年 / 15卷 / 04期
关键词：
real-time PCR; GeneXpert; M; tuberculosis; extra-pulmonary tuberculosis; ACID AMPLIFICATION TESTS; EXTRAPULMONARY TUBERCULOSIS; DIAGNOSIS; PERFORMANCE; TECHNOLOGY; PCR;
D O I：
10.5588/ijtld.10.0497
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
The detection of Mycobacterium tuberculosis using a new commercial real-time polymerase chain reaction (PCR) assay (Xpert (TM) MTB/RIF) was evaluated on 91 respiratory and 89 non-respiratory samples recovered from 132 patients suspected of tuberculosis (TB). Overall, 31 (17.2%) of the 180 samples, including 17 respiratory and 14 non-respiratory (respectively 17 and 12 PCR-positive), yielded M. tuberculosis on culture. The sensitivity and specificity of PCR were respectively 100% and 100%, and 85.7% and 97.3% for respiratory and non-respiratory samples. Although the test is validated only for respiratory samples, our findings suggest that it could be useful for the diagnosis of extra-pulmonary TB.
引用
收藏
页码：553 / 555
页数：3
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