Profiling of defense responsive pathway regulatory genes in Asian rice (Oryza sativa) against infection of Meloidogyne graminicola (Nematoda:Meloidogynidae)

被引:12
|
作者
Hatzade, Bhupal [1 ]
Singh, Divya [1 ]
Phani, Victor [1 ,2 ]
Kumbhar, Shailesh [3 ]
Rao, Uma [1 ]
机构
[1] ICAR Indian Agr Res Inst, Div Nematol, New Delhi, India
[2] Uttar Banga Krishi Viswavidyalaya, Coll Agr, Dakshin Dinajpur, W Bengal, India
[3] Mahatma Phule Krishi Vidyapeeth, Agr Res Stn, Rahuri, Maharashtra, India
关键词
Rice; Meloidogyne graminicola; Plant defense; Resistance; Susceptible; ROOT-KNOT NEMATODE; PATHOGENESIS-RELATED GENES; SALICYLIC-ACID; DISEASE RESISTANCE; JASMONIC ACID; CYST-NEMATODE; SIGNAL-TRANSDUCTION; PLANT; EXPRESSION; TOMATO;
D O I
10.1007/s13205-020-2055-3
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
In the present study, 36 Asian rice cultivars/landraces were evaluated against M. graminicola under in vitro conditions using soilless Pluronic gel medium. The cultivars/genotypes Phule Radha, EK 70, LK 248 and Khalibagh showed significantly reduced nematode infection, endoparasitic development, and derived multiplication factor indicating the presence of resistance, while Halvi Sal 17 was found to be most susceptible. Performance of selected genotypes showing resistance/susceptibility under in vitro conditions was further confirmed in soil which also revealed Phule Radha to be highly resistant and Halvi Sal 17 as the most susceptible genotype. Further, expression profile of plant defense responsive genes related to MAPK pathway, phytohormones, PR-proteins and callose and lignin synthesis were quantified in Phule Radha (the most resistant) and Halvi Sal 17 (the most susceptible) at 2 and 6 days post nematode inoculation. Significant upregulated expression of several defensive genes was observed in the resistant cultivar Phule Radha in contrast to insignificant expression in the susceptible varieties. The resistant genotype identified in the present study will be highly promising for resistance breeding in rice against M. graminicola.
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页数:13
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