Background Lifestyle modification in healthy workers is challenging. We aim to investigate associations between job stress and healthy behavior change among workers. Methods This cross-sectional study investigated 1,183 Japanese male white-collar workers in 2008 during health checkups for Metabolic Syndrome. Healthy behavior included either a calorie-focused diet or regular exercise. Job stress was measured by Job Content Questionnaire based on the job demands-control model and tension-anxiety and anger-hostility scales on the Profile of Mood States. Results Healthy behaviors were confirmed in 54% of study subjects. Multivariate logistic model showed that healthy behaviors were positively associated with a higher degree of work control and negatively associated with greater work demand. Work control and support were negatively correlated with tension-anxiety and depression, whereas work demand and strain were positively correlated with these two emotion domains (all P's < 0.0001). Conclusions It is suggested that addressing job stress is of clinical importance to promote healthy behaviors. Am. J. Ind. Med. 53:1128-1134, 2010. (c) 2010 Wiley-Liss, Inc.