Preparation, characterization and in vitro release study of BSA-loaded double-walled glucose-poly(lactide-co-glycolide) microspheres

被引:12
|
作者
Ansary, Rezaul H. [1 ]
Rahman, Mokhlesur M. [1 ]
Awang, Mohamed B. [2 ]
Katas, Haliza [3 ]
Hadi, Hazrina [1 ]
Mohamed, Farahidah [1 ]
Doolaanea, Abd Almonem [1 ]
Kamaruzzaman, Yunus B. [4 ]
机构
[1] IIUM, Kulliyyah Pharm, Kuantan 25200, Malaysia
[2] Cyberjaya Univ, Fac Pharm, Coll Med Sci, Cyberjaya 63000, Malaysia
[3] Univ Kebangsaan Malaysia, Drug Delivery & Novel Targeting Res Grp, Ctr Drug Delivery Res, Fac Pharm, Kuala Lumpur 50300, Malaysia
[4] Int Islamic Univ Malaysia, Kulliyyah Sci, Kuantan 25200, Malaysia
关键词
Drug delivery; Controlled release; Encapsulation efficiency; Microspheres; Poly(lactide-co-glycolide); Therapeutic proteins; PROTEIN RELEASE; DRUG-RELEASE; POLY(LACTIDE-CO-GLYCOLIDE) MICROSPHERES; BIODEGRADABLE MICROSPHERES; ENCAPSULATION EFFICIENCY; POLYMER PROPERTIES; SUSTAINED-RELEASE; MICROPARTICLES; NANOPARTICLES; DELIVERY;
D O I
10.1007/s12272-016-0710-3
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
The aim of this study was to prepare a model protein, bovine serum albumin (BSA) loaded double-walled microspheres using a fast degrading glucose core, hydroxyl-terminated poly(lactide-co-glycolide) (Glu-PLGA) and a moderate-degrading carboxyl-terminated PLGA polymers to reduce the initial burst release and to eliminate the lag phase from the release profile of PLGA microspheres. The double-walled microspheres were prepared using a modified water-in-oil-in-oil-in-water (w/o/o/w) method and single-polymer microspheres were prepared using a conventional water-in-oil-in-water (w/o/w) emulsion solvent evaporation method. The particle size, morphology, encapsulation efficiency, thermal properties, in vitro drug release and structural integrity of BSA were evaluated in this study. Double-walled microspheres prepared with Glu-PLGA and PLGA polymers with a mass ratio of 1:1 were non-porous, smooth-surfaced, and spherical in shape. A significant reduction of initial burst release was achieved for the double-walled microspheres compared to single-polymer microspheres. In addition, microspheres prepared using Glu-PLGA and PLGA polymers in a mass ratio of 1:1 exhibited continuous BSA release after the small initial burst without any lag phase. It can be concluded that the double-walled microspheres made of Glu-PLGA and PLGA polymers in a mass ratio of 1:1 can be a potential delivery system for pharmaceutical proteins.
引用
收藏
页码:1242 / 1256
页数:15
相关论文
共 50 条
  • [1] Preparation, characterization and in vitro release study of BSA-loaded double-walled glucose-poly(lactide-co-glycolide) microspheres
    Rezaul H. Ansary
    Mokhlesur M. Rahman
    Mohamed B. Awang
    Haliza Katas
    Hazrina Hadi
    Farahidah Mohamed
    Abd Almonem Doolaanea
    Yunus B. Kamaruzzaman
    Archives of Pharmacal Research, 2016, 39 : 1242 - 1256
  • [2] Preparation, characterization, and in vitro release studies of insulin-loaded double-walled poly(lactide-co-glycolide) microspheres
    Rezaul H. Ansary
    Mokhlesur M. Rahman
    Mohamed B. Awang
    Haliza Katas
    Hazrina Hadi
    Abd Almonen Doolaanea
    Drug Delivery and Translational Research, 2016, 6 : 308 - 318
  • [3] Preparation, characterization, and in vitro release studies of insulin-loaded double-walled poly(lactide-co-glycolide) microspheres
    Ansary, Rezaul H.
    Rahman, Mokhlesur M.
    Awang, Mohamed B.
    Katas, Haliza
    Hadi, Hazrina
    Doolaanea, Abd Almonen
    DRUG DELIVERY AND TRANSLATIONAL RESEARCH, 2016, 6 (03) : 308 - 318
  • [4] Controlled Release of Lysozyme from Double-Walled Poly(Lactide-Co-Glycolide) (PLGA) Microspheres
    Ansary, Rezaul H.
    Rahman, Mokhlesur M.
    Mohamad, Nasir
    Arrif, Tengku M.
    Latif, Ahmad Zubaidi A.
    Katas, Haliza
    Nik, Wan Sani B. Wan
    Awang, Mohamed B.
    POLYMERS, 2017, 9 (10):
  • [5] Preparation and characterization of poly(lactide-co-glycolide) microspheres for the sustained release of AZT
    Khang, G
    Lee, JH
    Lee, JW
    Cho, JC
    Lee, HB
    KOREA POLYMER JOURNAL, 2000, 8 (02): : 80 - 88
  • [6] In vitro Ciprofloxacin release from poly(lactide-co-glycolide) microspheres
    Martinez, B
    Lairion, F
    Pena, MB
    DiRocco, P
    Nacucchio, MC
    JOURNAL OF MICROENCAPSULATION, 1997, 14 (02) : 155 - 161
  • [7] PERCOLATION AND RELEASE OF CISPLATIN LOADED IN POLY(LACTIDE-CO-GLYCOLIDE) MICROSPHERES FOR CHEMOEMBOLIZATION
    DEYME, M
    SPENLEHAUER, G
    BENOIT, JP
    JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS, 1992, 7 (02) : 149 - 160
  • [8] Double-walled microspheres loaded with meglumine antimoniate: preparation, characterization and in vitro release study
    Navaei, Ali
    Rasoolian, Morteza
    Momeni, Arash
    Emami, Shahriar
    Rafienia, Mohammad
    DRUG DEVELOPMENT AND INDUSTRIAL PHARMACY, 2014, 40 (06) : 701 - 710
  • [9] Development and Characterization of Olanzapine Loaded Poly(lactide-co-glycolide) Microspheres for Depot Injection: In vitro and In vivo Release Profiles
    Pervaiz, Fahad
    Ahmad, Mahmood
    Li, Lihong
    Murtaza, Ghulam
    CURRENT DRUG DELIVERY, 2019, 16 (04) : 375 - 383
  • [10] Method matters: Development of characterization techniques for branched and glucose-poly(lactide-co-glycolide) polymers
    Hadar, Justin
    Skidmore, Sarah
    Garner, John
    Park, Haesun
    Park, Kinam
    Wang, Yan
    Qin, Bin
    Jiang, Xiaohui
    Kozak, Darby
    JOURNAL OF CONTROLLED RELEASE, 2020, 320 : 484 - 494