Screening Retinal Transplants with Fourier-domain OCT

被引:0
|
作者
Rao, Bin [1 ,3 ]
Yu, Lingfeng [3 ]
Aramant, Robert B. [4 ,5 ,6 ]
Pham, Sylvia [3 ]
Keirstead, Hans K. [4 ,5 ]
Chen, Zhongping [1 ,2 ,3 ]
Seiler, Magdalene J. [4 ,5 ]
机构
[1] Univ Calif Irvine, Dept Elect Engn & Comp Sci, Irvine, CA 92612 USA
[2] Univ Calif Irvine, Dept Biomed Engn, Irvine, CA 92612 USA
[3] Univ Calif Irvine, Beckman Laser Inst, Irvine, CA 92612 USA
[4] Univ Calif Irvine, Reeve Irvine Res Ctr, Irvine, CA 92612 USA
[5] Univ Calif Irvine, Sue & Bill Gross Stem Cell Res Ctr, Irvine, CA 92612 USA
[6] Univ Louisville, Dept Anat Sci & Neurobiol, Louisville, KY 40292 USA
关键词
D O I
10.1117/12.809547
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Transplant technology have been studied for the recovery of vision loss from retinitis pigmentosa (RP) and age-related macular degeneration (AMD). In several rodent retinal degeneration models and in patients, retinal progenitor cells transplanted as layers to the subretinal space have been shown to restore or preserve vision. The methods for evaluation of transplants are expensive considering the large amount of animals. Alternatively, time-domain Stratus OCT was previously shown to be able to image the morphological structure of transplants to some extent, but could not clearly identify laminated transplants. The efficacy of screening retinal transplants with Fourier-domain OCT was studied on 37 S334ter line 3 rats with retinal degeneration 6-67 days after transplant surgery. The transplants were morphologically categorized as no transplant, detachment, rosettes, small laminated area and larger laminated area with both Fourier-domain OCT and histology. The efficacy of Fourier-domain OCT in screening retinal transplants was evaluated by comparing the categorization results with OCT and histology. Additionally, 4 rats were randomly selected for multiple OCT examinations (1, 5, 9, 14 and 21days post surgery) in order to determine the earliest image time of OCT examination since the transplanted tissue may need some time to show its tendency of growing. Finally, we demonstrated the efficacy of Fourier-domain OCT in screening retinal transplants in early stages and determined the earliest imaging time for OCT. Fourier-domain OCT makes itself valuable in saving resource spent on animals with unsuccessful transplants.
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页数:5
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